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Moreover, in order to compare switch off times among the different experiments, we computed the defined as the time the circuit needed to achieve the of the mean initial fluorescence calculated for each experiment as follows 12 with fluorescence of the frame in the sequence smoothed by moving average filtering <a href=https://fastpriligy.top/>do i need a doctor prescription to buy priligy</a> RNA seq was performed using RNAs prepared from MCF 7 cells that were treated with E 2 for 0, 2, 4, 8, 12, and 24 h